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SNAI2 ELISA Kit (Rat)

Selected Variant

OKEH06017-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Snail family transcriptional repressor 2

Gene Aliases

Neural crest transcription factor Slug; protein snail homolog 2; Slug; Slug, chicken homolog; snail family zinc finger 2; snail homolog 2; zinc finger protein SNAI2.

Gene ID

25554

Accession Number

NP_037167.1

Reactivity

Rat, Rattus norvegicus

Target

Zinc finger protein SNAI2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Snai2 ELISA Kit (Rat) (OKEH06017) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Snai2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Snai2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Snai2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 93%

Sensitivity

0.087 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Zinc finger protein SNAI2

Protein Length

268

NCBI Gene Symbol

Snai2

Protein Name

O08954

Gene Name URL

Snai2

Nucleotide Accession Number

NM_013035.1

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