CAMKK2 ELISA Kit (Rat)
Selected Variant
OKEH06015-96W
Size: 96 Well
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Calcium/calmodulin-dependent protein kinase kinase 2
Gene Aliases
Ca+/Calmodulin-dependent protein kinase kinase beta (CaM-kinase kinase beta) ; calcium/calmodulin-dependent protein kinase 2 beta; calcium/calmodulin-dependent protein kinase kinase 2; calcium/calmodulin-dependent protein kinase kinase 2, beta; calcium/calmodulin-dependent protein kinase kinase beta; caM-kinase kinase 2; CaM-kinase kinase beta; caMKK 2; caM-KK 2; caMKK beta; caM-KK beta.
Gene ID
83506
Accession Number
NP_112628.1
Reactivity
Rat, Rattus norvegicus
Target
Calcium/calmodulin-dependent protein kinase kinase 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Camkk2 ELISA Kit (Rat) (OKEH06015) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Camkk2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Camkk2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Camkk2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 90%
Sensitivity
0.329 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Calcium/calmodulin-dependent protein kinase kinase 2
Protein Length
587
NCBI Gene Symbol
Camkk2
Protein Name
O88831
Gene Name URL
Camkk2
Nucleotide Accession Number
NM_031338.1
Price
Sign In for Pricing
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog