KIRREL ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Kirre like nephrin family adhesion molecule 1
Gene Aliases
Kin of IRRE like 1; kin of irregular chiasm-like protein 1; kin of IRRE-like protein 1; Kirrel; Neph1; nephrin 1; nephrin-like protein 1.
Gene ID
310695
Accession Number
NP_997489.1
Reactivity
Rat, Rattus norvegicus
Target
Kin of IRRE-like protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Kirrel ELISA Kit (Rat) (OKEH06010) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Kirrel has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Kirrel is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Kirrel captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 99%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Kin of IRRE-like protein 1
Protein Length
789
NCBI Gene Symbol
Kirrel1
Protein Name
Q6X936
Gene Name URL
Kirrel1
Nucleotide Accession Number
NM_207606.1
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