LYPD3 ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
Gene Name
Ly6/Plaur domain containing 3
Gene Aliases
C4.4a; GPI-anchored metastasis-associated protein C4.4A; GPI-anchored metastasis-associated protein homolog; ly6/PLAUR domain-containing protein 3; metastasis-associated GPI-anchored protein; metastasis-associated molecule.
Gene ID
60378
Accession Number
NP_068527.1
Reactivity
Rat, Rattus norvegicus
Target
Ly6/PLAUR domain-containing protein 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Lypd3 ELISA Kit (Rat) (OKEH06009) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lypd3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Lypd3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lypd3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 91%
Sensitivity
0.16 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Ly6/PLAUR domain-containing protein 3
Protein Length
352
NCBI Gene Symbol
Lypd3
Protein Name
O55162
Gene Name URL
Lypd3
CAS Number
7732-18-5
Nucleotide Accession Number
NM_021759.1
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