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SORBS1 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Sorbin and SH3 domain-containing protein 1

Gene Aliases

Ponsin; SH3P12.

Gene ID

309487

Accession Number

XP_017445843.1

Reactivity

Rat, Rattus norvegicus

Target

Sorbin and SH3 domain-containing protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Sorbs1 ELISA Kit (Rat) (OKEH05996) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sorbs1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Sorbs1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sorbs1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.625-40ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 94%

Sensitivity

0.333 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Sorbin and SH3 domain-containing protein 1

Protein Length

35

NCBI Gene Symbol

SORBS1

Protein Name

P84109

Gene Name URL

SORBS1

Nucleotide Accession Number

XM_017590354.1

Frequently Asked Questions

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