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DNAJC27 ELISA Kit (Rat)

Selected Variant

OKEH05994-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

DnaJ heat shock protein family (Hsp40) member C27

Gene Aliases

DnaJ (Hsp40) homolog, subfamily C, member 27; dnaJ homolog subfamily C member 27; rab and DnaJ domain containing; rab and DnaJ domain-containing protein; Rbj.

Gene ID

298859

Accession Number

NP_996727.2

Reactivity

Rat, Rattus norvegicus

Target

DnaJ homolog subfamily C member 27

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Dnajc27 ELISA Kit (Rat) (OKEH05994) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dnajc27 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Dnajc27 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dnajc27 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 86%

Sensitivity

0.083 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat DnaJ homolog subfamily C member 27

Protein Length

273

NCBI Gene Symbol

Dnajc27

Protein Name

Q6IML7

Gene Name URL

Dnajc27

Nucleotide Accession Number

NM_206845.2

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