HBP1 ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
HMG-box transcription factor 1
Gene Aliases
High mobility group box transcription factor 1; high mobility group protein 1; HMG box transcription factor 1; HMG box-containing protein 1; Hmgb1.
Gene ID
27080
Accession Number
NP_037353.2
Reactivity
Rat, Rattus norvegicus
Target
HMG box-containing protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Hbp1 ELISA Kit (Rat) (OKEH05978) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hbp1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Hbp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hbp1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 86%
Sensitivity
0.157 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat HMG box-containing protein 1
Protein Length
513
NCBI Gene Symbol
Hbp1
Protein Name
Q62661
Gene Name URL
Hbp1
Nucleotide Accession Number
NM_013221.2
Frequently Asked Questions
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