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HBP1 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

HMG-box transcription factor 1

Gene Aliases

High mobility group box transcription factor 1; high mobility group protein 1; HMG box transcription factor 1; HMG box-containing protein 1; Hmgb1.

Gene ID

27080

Accession Number

NP_037353.2

Reactivity

Rat, Rattus norvegicus

Target

HMG box-containing protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Hbp1 ELISA Kit (Rat) (OKEH05978) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hbp1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Hbp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hbp1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into Serum and Plasma = 86%

Sensitivity

0.157 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat HMG box-containing protein 1

Protein Length

513

NCBI Gene Symbol

Hbp1

Protein Name

Q62661

Gene Name URL

Hbp1

Nucleotide Accession Number

NM_013221.2

Frequently Asked Questions

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