PIP4K2C ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Phosphatidylinositol-5-phosphate 4-kinase type 2 gamma
Gene Aliases
Pip5k2c
Gene ID
140607
Accession Number
NP_536728.2
Reactivity
Rat
Target
May play an important role in the production of Phosphatidylinositol bisphosphate (PIP2), in the endoplasmic reticulum.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Pip4k2c ELISA Kit (Rat) (OKEH05977) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pip4k2c has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Pip4k2c is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pip4k2c captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156 - 10ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 89%
Sensitivity
0.085 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma
Protein Length
420
NCBI Gene Symbol
PIP4K2C
Protein Name
Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma
Gene Name URL
PIP4K2C
Nucleotide Accession Number
NM_080480.2
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