PARM1 ELISA Kit (Rat)
Selected Variant
OKEH05975-96W
Size: 96 Tests
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Prostate androgen-regulated mucin-like protein 1
Gene Aliases
Cipar1, PARM-1, CIPAR-1
Gene ID
286894
Accession Number
NP_775137.1
Reactivity
Rat
Target
May regulate TLP1 expression and telomerase activity, thus enabling certain prostatic cells to resist apoptosis.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Parm1 ELISA Kit (Rat) (OKEH05975) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Parm1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Parm1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Parm1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.56 - 100ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 104%
Sensitivity
0.78 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Prostate androgen-regulated mucin-like protein 1 homolog
Protein Length
296
NCBI Gene Symbol
PARM1
Protein Name
Prostate androgen-regulated mucin-like protein 1 homolog
Gene Name URL
PARM1
Nucleotide Accession Number
NM_173114.1
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