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PARM1 ELISA Kit (Rat)

Selected Variant

OKEH05975-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Prostate androgen-regulated mucin-like protein 1

Gene Aliases

Cipar1, PARM-1, CIPAR-1

Gene ID

286894

Accession Number

NP_775137.1

Reactivity

Rat

Target

May regulate TLP1 expression and telomerase activity, thus enabling certain prostatic cells to resist apoptosis.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Parm1 ELISA Kit (Rat) (OKEH05975) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Parm1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Parm1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Parm1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56 - 100ng/ml

Recovery

Mean recovery when spiking into Serum and Plasma = 104%

Sensitivity

0.78 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Prostate androgen-regulated mucin-like protein 1 homolog

Protein Length

296

NCBI Gene Symbol

PARM1

Protein Name

Prostate androgen-regulated mucin-like protein 1 homolog

Gene Name URL

PARM1

Nucleotide Accession Number

NM_173114.1

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