TCAF3 ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Family with sequence similarity 115, member E
Gene Aliases
Eapa2, Tcaf3
Gene ID
494321
Accession Number
NP_001009534.1
Reactivity
Rat
Target
May play a role in the regulation of the cation channel TRPM8 activity.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Tcaf3 ELISA Kit (Rat) (OKEH05968) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tcaf3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Tcaf3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tcaf3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312 - 20ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 95%
Sensitivity
0.18 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Experimental autoimmune prostatitis antigen 2 homolog
Protein Length
914
NCBI Gene Symbol
TCAF3
Protein Name
TRPM8 channel-associated factor 3
Gene Name URL
TCAF3
Nucleotide Accession Number
NM_001009534.1
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
