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SLC27A5 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Solute carrier family 27 member 5

Gene Aliases

BA-CoA ligase; BACS; BAL; bile acid-CoA ligase; bile acyl-CoA synthetase; cholate--CoA ligase; FATP-5; fatty acid transport protein 5; rBAL-1; solute carrier family 27 (fatty acid transporter), member 5; Solute carrier family 27 member 5; very long-chain acyl-CoA synthetase-related protein; VLACSR; VLACS-related.

Gene ID

79111

Accession Number

NP_077057.1

Reactivity

Rat, Rattus norvegicus

Target

Bile acyl-CoA synthetase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Slc27a5 ELISA Kit (Rat) (OKEH05954) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc27a5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Slc27a5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc27a5 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 96%

Sensitivity

39.5 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Bile acyl-CoA synthetase

Protein Length

690

NCBI Gene Symbol

Slc27a5

Protein Name

Q9ES38

Gene Name URL

Slc27a5

Nucleotide Accession Number

NM_024143.2

Frequently Asked Questions

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