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BCAM ELISA Kit (Rat)

Selected Variant

OKEH05952-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Basal cell adhesion molecule (Lutheran blood group)

Gene Aliases

Basal cell adhesion molecule; B-CAM cell surface glycoprotein; Lu; lutheran antigen; Lutheran blood group (Auberger b antigen included) .

Gene ID

78958

Accession Number

NP_113940.1

Reactivity

Rat, Rattus norvegicus

Target

Basal cell adhesion molecule

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Bcam ELISA Kit (Rat) (OKEH05952) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bcam has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Bcam is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bcam captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 85%

Sensitivity

39.7 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Basal cell adhesion molecule

Protein Length

624

NCBI Gene Symbol

Bcam

Protein Name

Q9ESS6

Gene Name URL

Bcam

Nucleotide Accession Number

NM_031752.2

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