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APOC4 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Apolipoprotein C4

Gene Aliases

Acl; apoC-IV; apo-CIV; apolipoprotein C2 linked; Apolipoprotein C4; apolipoprotein CIV; apolipoprotein C-IV; apolipoprotein E-linked; ECL.

Gene ID

680551

Accession Number

NP_001102889.1

Reactivity

Rat, Rattus norvegicus

Target

Apolipoprotein C-IV

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Apoc4 ELISA Kit (Rat) (OKEH05949) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apoc4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Apoc4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apoc4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 85%

Sensitivity

7.92 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Apolipoprotein C-IV

Protein Length

124

NCBI Gene Symbol

Apoc4

Protein Name

P55797

Gene Name URL

Apoc4

Nucleotide Accession Number

NM_001109419.1

Frequently Asked Questions

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