ALDH1A7 ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Aldehyde dehydrogenase, cytosolic 1
Gene Aliases
Aldhpb, Aldh1a4
Gene ID
29651
Accession Number
NP_058968.14
Reactivity
Rat
Target
Can oxidize benzaldehyde, propionaldehyde and acetaldehyde. No detectable activity with retinal.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Aldh1a7 ELISA Kit (Rat) (OKEH05935) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Aldh1a7 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Aldh1a7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Aldh1a7 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78 - 5000pg/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 81%
Sensitivity
40.6 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Aldehyde dehydrogenase, cytosolic 1
Protein Length
501
NCBI Gene Symbol
ALDH1A7
Protein Name
Aldehyde dehydrogenase, cytosolic 1
Gene Name URL
ALDH1A7
Nucleotide Accession Number
NM_017272.15
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