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MANBA ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Mannosidase, beta A, lysosomal

Gene Aliases

2410030O07Rik; B930014J03Rik; beta-mannosidase; Bm; Bmn; lysosomal beta A mannosidase; mannanase; mannase.

Gene ID

110173

Accession Number

NP_081564.3

Reactivity

Mouse, Mus musculus

Target

Beta-mannosidase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Manba ELISA Kit (Mouse) (OKEH05907) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Manba has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Manba is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Manba captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 89.2%

Sensitivity

15.6 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Beta-mannosidase

Protein Length

879

NCBI Gene Symbol

Manba

Protein Name

Q8K2I4

Gene Name URL

Manba

Nucleotide Accession Number

NM_027288.3

Frequently Asked Questions

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