ITGB2 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Integrin beta 2
Gene Aliases
2E6; AI528527; Cd18; cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement receptor C3 subunit beta; integrin beta-2; LAD; LCAMB; Lfa1; lymphocyte function associated antigen 1; Mac-1 beta; macrophage antigen-1 beta; MF17.
Gene ID
16414
Accession Number
NP_032430.2
Reactivity
Mouse, Mus musculus
Target
Integrin beta-2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Itgb2 ELISA Kit (Mouse) (OKEH05898) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Itgb2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Itgb2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Itgb2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 88.4%
Sensitivity
0.404 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Integrin beta-2
Protein Length
771
NCBI Gene Symbol
Itgb2
Protein Name
P11835
Gene Name URL
Itgb2
Nucleotide Accession Number
NM_008404.4
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