What is the catalog number for TRIM21 ELISA Kit (Human)?

The catalog number for TRIM21 ELISA Kit (Human) is OKEH05877-96W.

What sizes are available for TRIM21 ELISA Kit (Human)?

TRIM21 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is TRIM21 ELISA Kit (Human) in stock?

TRIM21 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

TRIM21 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Tripartite motif containing 21

Gene Aliases

52 kDa ribonucleoprotein autoantigen Ro/SS-A;52 kDa Ro protein; E3 ubiquitin-protein ligase TRIM21; RING finger protein 81; RING-type E3 ubiquitin transferase TRIM21; RNF81; ro (SS-A) ; Ro/SSA; Ro/SSA 52kDa; RO52; Sicca syndrome antigen A; sjoegren syndrome type A antigen; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro) ; SSA; SS-A; SSA1; tripartite motif-containing protein 21.

Gene ID

6737

Accession Number

NP_003132.2

Reactivity

Homo sapiens, Human

Target

E3 ubiquitin-protein ligase TRIM21

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TRIM21 ELISA Kit (Human) (OKEH05877) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TRIM21 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for TRIM21 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TRIM21 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol