INTS3 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Integrator complex subunit 3
Gene Aliases
C77668; int3; integrator complex subunit 3; sensor of single-strand DNA complex subunit A; sensor of ssDNA subunit A; SOSS complex subunit A; SOSS-A.
Gene ID
229543
Accession Number
NP_663515.2
Reactivity
Mouse, Mus musculus
Target
Integrator complex subunit 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ints3 ELISA Kit (Mouse) (OKEH05875) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ints3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ints3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ints3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 89.9%
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Integrator complex subunit 3
Protein Length
1041
NCBI Gene Symbol
Ints3
Protein Name
Q7TPD0
Gene Name URL
Ints3
Nucleotide Accession Number
NM_145540.3
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