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C1QC ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Complement component 1, q subcomponent, C chain

Gene Aliases

Adib; adiponectin b; AI385742; C1qg; Ciqc; complement C1q subcomponent subunit C; complement component 1, q subcomponent, c polypeptide; complement component 1, q subcomponent, gamma polypeptide.

Gene ID

12262

Accession Number

NP_031600.2

Reactivity

Mouse, Mus musculus

Target

Complement C1q subcomponent subunit C

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology C1qc ELISA Kit (Mouse) (OKEH05854) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C1qc has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for C1qc is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C1qc captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 98.4%

Sensitivity

0.082 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Complement C1q subcomponent subunit C

Protein Length

246

NCBI Gene Symbol

C1qc

Protein Name

Q02105

Gene Name URL

C1qc

Nucleotide Accession Number

NM_007574.2

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