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ENOX1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Ecto-NOX disulfide-thiol exchanger 1

Gene Aliases

BA64J21.1; candidate growth-related and time keeping constitutive hydroquinone (NADH) oxidase; candidate growth-related and time keeping constitutive hydroquinone [NADH] oxidase; cCNOX; cell proliferation-inducing gene 38 protein; CNOX; constitutive Ecto-NOX; ecto-NADPH oxidase disulfide-thiol exchanger 1; ecto-NOX disulfide-thiol exchanger 1; PIG38.

Gene ID

55068

Accession Number

NP_001121087.1

Reactivity

Homo sapiens, Human

Target

Ecto-NOX disulfide-thiol exchanger 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ENOX1 ELISA Kit (Human) (OKEH05842) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ENOX1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for ENOX1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ENOX1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

3.12-200pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 106%

Sensitivity

1.69 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Ecto-NOX disulfide-thiol exchanger 1

Protein Length

643

NCBI Gene Symbol

ENOX1

Protein Name

Q8TC92

Gene Name URL

ENOX1

Nucleotide Accession Number

NM_001127615.1

Frequently Asked Questions

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