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DPP10 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Dipeptidylpeptidase 10

Gene Aliases

6430601K09Rik; dipeptidyl peptidase X; DP; DPP X; Dprp3; inactive dipeptidyl peptidase 10.

Gene ID

269109

Accession Number

NP_950186.3

Reactivity

Mouse, Mus musculus

Target

Inactive dipeptidyl peptidase 10

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Dpp10 ELISA Kit (Mouse) (OKEH05815) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dpp10 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Dpp10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dpp10 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 91.9%

Sensitivity

0.169 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Inactive dipeptidyl peptidase 10

Protein Length

797

NCBI Gene Symbol

Dpp10

Protein Name

Q6NXK7

Gene Name URL

Dpp10

Nucleotide Accession Number

NM_199021.3

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