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NUSAP1 ELISA Kit (Mouse)

Selected Variant

OKEH05806-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Nucleolar and spindle associated protein 1

Gene Aliases

2610201A12Rik; AI481307; ANKT; AW547774; BB165529; BM037; LNP; N; nucleolar and spindle-associated protein 1; nucleolar protein ANKT; NuSAP; Q0310; SAPL.

Gene ID

108907

Accession Number

NP_001036117.1

Reactivity

Mouse, Mus musculus

Target

Nucleolar and spindle-associated protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Nusap1 ELISA Kit (Mouse) (OKEH05806) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nusap1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Nusap1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nusap1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 85.8%

Sensitivity

39.5 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Nucleolar and spindle-associated protein 1

Protein Length

427

NCBI Gene Symbol

Nusap1

Protein Name

Q9ERH4

Gene Name URL

Nusap1

Nucleotide Accession Number

NM_001042652.1

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