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SMAD1 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

SMAD family member 1

Gene Aliases

AI528653; dwarfin-A; dwf-A; Mad; MAD homolog 1; Mad1; Madh; Madh1; Madr1; mad-related protein 1; Mlp1; mMad1; mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; mothers-against-DPP-related 1; MusMLP; SMAD family member 1.

Gene ID

17125

Accession Number

NP_032565.2

Reactivity

Mouse, Mus musculus

Target

Mothers against decapentaplegic homolog 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Smad1 ELISA Kit (Mouse) (OKEH05787) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Smad1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Smad1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Smad1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 86.8%

Sensitivity

31.34 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Mothers against decapentaplegic homolog 1

Protein Length

465

NCBI Gene Symbol

Smad1

Protein Name

P70340

Gene Name URL

Smad1

Nucleotide Accession Number

NM_008539.3

Frequently Asked Questions

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