SMAD1 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
SMAD family member 1
Gene Aliases
AI528653; dwarfin-A; dwf-A; Mad; MAD homolog 1; Mad1; Madh; Madh1; Madr1; mad-related protein 1; Mlp1; mMad1; mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; mothers-against-DPP-related 1; MusMLP; SMAD family member 1.
Gene ID
17125
Accession Number
NP_032565.2
Reactivity
Mouse, Mus musculus
Target
Mothers against decapentaplegic homolog 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Smad1 ELISA Kit (Mouse) (OKEH05787) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Smad1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Smad1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Smad1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
62.5-4000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 86.8%
Sensitivity
31.34 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Mothers against decapentaplegic homolog 1
Protein Length
465
NCBI Gene Symbol
Smad1
Protein Name
P70340
Gene Name URL
Smad1
Nucleotide Accession Number
NM_008539.3
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
MBS6006153-01 0.1 mg
HCST (DAP10, Hematopoietic Cell Signal Transducer, DNAX-activation Protein 10, Membrane Protein DAP10, Transmembrane Adapter Protein KAP10, KAP10, PIK3AP, UNQ587/PRO1157)
Sign In for Pricing
View Details MBS6006153-02 5x 0.1 mg
HCST (DAP10, Hematopoietic Cell Signal Transducer, DNAX-activation Protein 10, Membrane Protein DAP10, Transmembrane Adapter Protein KAP10, KAP10, PIK3AP, UNQ587/PRO1157)
Sign In for Pricing
View Details 
MBS6241425-01 0.1 mL
ZNF274 (Zinc Finger Protein 274, DKFZp686K08243, FLJ37843, HFB101, ZF2, ZKSCAN19) (MaxLight 750)
Sign In for Pricing
View Details