TSPAN7 ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Tetraspanin 7
Gene Aliases
1200014P11Rik; A1; A15; AI323365; Cd231; cell surface glycoprotein A15; Mxs1; PE3; PE31; R74651; T; TALLA; TALLA homolog; tetraspanin-7; Tm4sf; Tm4sf2; transmembrane 4 superfamily member 2; tspan-7.
Gene ID
21912
Accession Number
NP_062608.2
Reactivity
Mouse, Mus musculus
Target
Tetraspanin-7
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tspan7 ELISA Kit (Mouse) (OKEH05774) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tspan7 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Tspan7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tspan7 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 107.8%
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Tetraspanin-7
Protein Length
249
NCBI Gene Symbol
Tspan7
Protein Name
Q62283
Gene Name URL
Tspan7
Nucleotide Accession Number
NM_019634.2
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