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IL20RA ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Interleukin 20 receptor, alpha

Gene Aliases

E230031K19; IL-20 receptor subunit alpha; IL-20R1; IL-20RA; IL-20R-alpha; interleukin-20 receptor subunit alpha.

Gene ID

237313

Accession Number

NP_766374.1

Reactivity

Mouse, Mus musculus

Target

Interleukin-20 receptor subunit alpha

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Il20ra ELISA Kit (Mouse) (OKEH05755) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il20ra has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Il20ra is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Il20ra captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 86.1%

Sensitivity

0.08 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Interleukin-20 receptor subunit alpha

Protein Length

546

NCBI Gene Symbol

Il20ra

Protein Name

Q6PHB0

Gene Name URL

Il20ra

Nucleotide Accession Number

NM_172786.2

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