KRT6A ELISA Kit (Mouse)
Selected Variant
OKEH05753-96W
Size: 96 Tests
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Keratin 6A
Gene Aliases
2310016L08Rik;60-kDa keratin; CK-6A; cytokeratin-6A; K6[a]; K6A; K6-alpha; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6c; keratin, type II cytoskeletal 6A; Keratin-6A; keratin-6-alpha; Krt2-; Krt2-6; Krt2-6a; Krt2-6c; MK6; mK6[a; mK6[a]; MK6a; mK6-alpha.
Gene ID
16687
Accession Number
NP_032502.3
Reactivity
Mouse, Mus musculus
Target
Keratin, type II cytoskeletal 6A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Krt6a ELISA Kit (Mouse) (OKEH05753) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Krt6a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Krt6a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Krt6a captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 100.4%
Sensitivity
39.4 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Keratin, type II cytoskeletal 6A
Protein Length
553
NCBI Gene Symbol
Krt6a
Protein Name
P50446
Gene Name URL
Krt6a
Nucleotide Accession Number
NM_008476.3
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