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KRT6A ELISA Kit (Mouse)

Selected Variant

OKEH05753-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Keratin 6A

Gene Aliases

2310016L08Rik;60-kDa keratin; CK-6A; cytokeratin-6A; K6[a]; K6A; K6-alpha; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6c; keratin, type II cytoskeletal 6A; Keratin-6A; keratin-6-alpha; Krt2-; Krt2-6; Krt2-6a; Krt2-6c; MK6; mK6[a; mK6[a]; MK6a; mK6-alpha.

Gene ID

16687

Accession Number

NP_032502.3

Reactivity

Mouse, Mus musculus

Target

Keratin, type II cytoskeletal 6A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Krt6a ELISA Kit (Mouse) (OKEH05753) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Krt6a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Krt6a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Krt6a captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 100.4%

Sensitivity

39.4 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Keratin, type II cytoskeletal 6A

Protein Length

553

NCBI Gene Symbol

Krt6a

Protein Name

P50446

Gene Name URL

Krt6a

Nucleotide Accession Number

NM_008476.3

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