PRKAA2 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Protein kinase, AMP-activated, alpha 2 catalytic subunit
Gene Aliases
2310008I11Rik;5'-AMP-activated protein kinase catalytic subunit alpha-2; A830082D05; ACACA kinase; acetyl-CoA carboxylase kinase; AMP-activated protein kinase alpha-2 variant B; AMPK; AMPK subunit alpha-2; AMPKalpha2; HMGCR kinase; hydroxymethylglutaryl-CoA reductase kinase.
Gene ID
108079
Accession Number
NP_835279.2
Reactivity
Mouse, Mus musculus
Target
5'-AMP-activated protein kinase catalytic subunit alpha-2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Prkaa2 ELISA Kit (Mouse) (OKEH05742) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prkaa2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Prkaa2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prkaa2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 110.9%
Sensitivity
39.6 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse 5'-AMP-activated protein kinase catalytic subunit alpha-2
Protein Length
552
NCBI Gene Symbol
Prkaa2
Protein Name
Q8BRK8
Gene Name URL
Prkaa2
Nucleotide Accession Number
NM_178143.2
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