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HTR1B ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

5-hydroxytryptamine (serotonin) receptor 1B

Gene Aliases

5-HT<1B> receptor;5-HT-1B;5-HT1B receptor;5-hydroxytryptamine receptor 1B; serotonin receptor 1B.

Gene ID

15551

Accession Number

NP_034612.1

Reactivity

Mouse, Mus musculus

Target

5-hydroxytryptamine receptor 1B

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Htr1b ELISA Kit (Mouse) (OKEH05737) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Htr1b has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Htr1b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Htr1b captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 95.2%

Sensitivity

0.163 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse 5-hydroxytryptamine receptor 1B

Protein Length

386

NCBI Gene Symbol

Htr1b

Protein Name

P28334

Gene Name URL

Htr1b

Nucleotide Accession Number

NM_010482.1

Frequently Asked Questions

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