MAP2K5 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Mitogen-activated protein kinase kinase 5
Gene Aliases
AI324775; AI428457; dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5; MAPK/ERK kinase 5; Mapkk5; MEK5; Prkmk5.
Gene ID
23938
Accession Number
NP_035970.1
Reactivity
Mouse, Mus musculus
Target
Dual specificity mitogen-activated protein kinase kinase 5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Map2k5 ELISA Kit (Mouse) (OKEH05731) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Map2k5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Map2k5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Map2k5 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 89.1%
Sensitivity
0.314 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Dual specificity mitogen-activated protein kinase kinase 5
Protein Length
448
NCBI Gene Symbol
Map2k5
Protein Name
Q9WVS7
Gene Name URL
Map2k5
Nucleotide Accession Number
NM_011840.2
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