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ACADM ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Acyl-CoA dehydrogenase medium chain

Gene Aliases

ACAD1; acyl-CoA dehydrogenase, C-4 to C-12 straight chain; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain; MCAD; MCADH; medium-chain acyl-CoA dehydrogenase; medium-chain specific acyl-CoA dehydrogenase, mitochondrial; testicular tissue protein Li 7.

Gene ID

34

Accession Number

NP_000007.1

Reactivity

Homo sapiens, Human

Target

Medium-chain specific acyl-CoA dehydrogenase, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ACADM ELISA Kit (Human) (OKEH05727) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACADM has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for ACADM is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACADM captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 99%

Sensitivity

40.1 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Medium-chain specific acyl-CoA dehydrogenase, mitochondrial

Protein Length

421

NCBI Gene Symbol

ACADM

Protein Name

P11310

Gene Name URL

ACADM

Nucleotide Accession Number

NM_000016.5

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