What is the catalog number for ECH1 ELISA Kit (Mouse)?

The catalog number for ECH1 ELISA Kit (Mouse) is OKEH05725-96W.

What sizes are available for ECH1 ELISA Kit (Mouse)?

ECH1 ELISA Kit (Mouse) is available in the following size(s): 96 Tests.

Is ECH1 ELISA Kit (Mouse) in stock?

ECH1 ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

ECH1 ELISA Kit (Mouse)

Selected Variant

OKEH05725-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Enoyl coenzyme A hydratase 1, peroxisomal

Gene Aliases

AA617331; delta (3,5) -Delta (2,4) -dienoyl-CoA isomerase, mitochondrial; dienoyl-CoA isomerase; peroxisomal/mitochondrial dienoyl-CoA isomerase.

Gene ID

51798

Accession Number

NP_058052.1

Reactivity

Mouse, Mus musculus

Target

Delta (3,5) -Delta (2,4) -dienoyl-CoA isomerase, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology Ech1 ELISA Kit (Mouse) (OKEH05725) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ech1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ech1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ech1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.78-50ng/ml

Recovery

Mean recovery when spiking into Serum and Plasma = 91.1%

Sensitivity

0.391 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Delta (3,5) -Delta (2,4) -dienoyl-CoA isomerase, mitochondrial

Protein Length

327

NCBI Gene Symbol

Ech1

Protein Name

O35459

Gene Name URL

Ech1

Nucleotide Accession Number

NM_016772.1

Price

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