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VTCN1 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

V-set domain containing T cell activation inhibitor 1

Gene Aliases

B7 homolog 4; B7h4; B7s1; B7x; BC032925; immune costimulatory protein B7-H4; Protein B7S1; t cell costimulatory molecule B7x; V-set domain containing T-cell activation inhibitor 1.

Gene ID

242122

Accession Number

NP_848709.2

Reactivity

Mouse, Mus musculus

Target

V-set domain containing T-cell activation inhibitor 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Vtcn1 ELISA Kit (Mouse) (OKEH05705) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Vtcn1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Vtcn1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Vtcn1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

3.12-200ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 103.8%

Sensitivity

1.76 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse V-set domain containing T-cell activation inhibitor 1

Protein Length

283

NCBI Gene Symbol

Vtcn1

Protein Name

Q7TSP5

Gene Name URL

Vtcn1

Nucleotide Accession Number

NM_178594.3

Frequently Asked Questions

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