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SLC29A1 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Solute carrier family 29 (nucleoside transporters), member 1

Gene Aliases

1200014D21Rik; AA407560; ENT; ENT1; equilibrative NBMPR-sensitive nucleoside transporter; equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; equilibrative nucleoside transporter 1; NBMPR-sensitive equilibrative nucleoside transporter; nucleoside transporter, es-type; solute carrier family 29 member 1.

Gene ID

63959

Accession Number

NP_001186042.1

Reactivity

Mouse, Mus musculus

Target

Equilibrative nucleoside transporter 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Slc29a1 ELISA Kit (Mouse) (OKEH05686) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc29a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Slc29a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc29a1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 88.4%

Sensitivity

0.08 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Equilibrative nucleoside transporter 1

Protein Length

460

NCBI Gene Symbol

Slc29a1

Protein Name

Q9JIM1

Gene Name URL

Slc29a1

Nucleotide Accession Number

NM_001199113.1

Frequently Asked Questions

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