What is the catalog number for HDAC8 ELISA Kit (Mouse)?

The catalog number for HDAC8 ELISA Kit (Mouse) is OKEH05680-96W.

What sizes are available for HDAC8 ELISA Kit (Mouse)?

HDAC8 ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is HDAC8 ELISA Kit (Mouse) in stock?

HDAC8 ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

Is there an additional fee for hazardous or dry ice shipping of this product?

Yes, if this product requires dry ice or is classed as hazardous for transport, the additional fee will be explicitly shown in your quotation before you confirm the order, so there are no surprise charges later.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

HDAC8 ELISA Kit (Mouse)

Selected Variant

OKEH05680-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Histone deacetylase 8

Gene Aliases

2610007D20Rik; HD8; histone deacetylase 8.

Gene ID

70315

Accession Number

NP_001300671.1

Reactivity

Mouse, Mus musculus

Target

Histone deacetylase 8

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Hdac8 ELISA Kit (Mouse) (OKEH05680) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hdac8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Hdac8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hdac8 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.625-40ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 102.9%

Sensitivity

0.324 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Histone deacetylase 8

Protein Length

377

NCBI Gene Symbol

Hdac8

Protein Name

Q8VH37

Gene Name URL

Hdac8

Nucleotide Accession Number

NM_001313742.1

Price

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