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ATP1A3 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

ATPase Na+/K+ transporting subunit alpha 3

Gene Aliases

AHC2; ATP1A1; ATPase, Na+/K+ transporting, alpha 3 polypeptide; CAPOS; DYT12; Na (+) /K (+) ATPase alpha (III) subunit; Na (+) /K (+) ATPase alpha-3 subunit; Na+, K+ activated adenosine triphosphatase alpha subunit; Na+/K+ ATPase 3; RDP; sodium pump subunit alpha-3; sodium/potassium-transporting ATPase alpha-3 chain; sodium/potassium-transporting ATPase subunit alpha-3; sodium-potassium ATPase catalytic subunit alpha-3; sodium-potassium-ATPase, alpha 3 polypeptide.

Gene ID

478

Accession Number

NP_001243142.1

Reactivity

Homo sapiens, Human

Target

Sodium/potassium-transporting ATPase subunit alpha-3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ATP1A3 ELISA Kit (Human) (OKEH05672) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATP1A3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for ATP1A3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATP1A3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.2-2000pg/ml

Recovery

Mean recovery when spiking into Serum and Plasma = 94%

Sensitivity

15.62 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Sodium/potassium-transporting ATPase subunit alpha-3

Protein Length

1013

NCBI Gene Symbol

ATP1A3

Protein Name

P13637

Gene Name URL

ATP1A3

Nucleotide Accession Number

NM_001256213.1

Frequently Asked Questions

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