ALDH5A1 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Aldhehyde dehydrogenase family 5, subfamily A1
Gene Aliases
6330403E24Rik; Ahd1; Ahd-1; aldehyde dehydrogenase 1, mitochondrial; aldehyde dehydrogenase family 5 member A1; aldehyde dehydrogenase family 5, subfamily A1; D630032B01Rik; NAD (+) -dependent succinic semialdehyde dehydrogenase; SS; SSADH; Ssdh1; succinate-semialdehyde dehydrogenase, mitochondrial.
Gene ID
214579
Accession Number
NP_766120.1
Reactivity
Mouse, Mus musculus
Target
Succinate-semialdehyde dehydrogenase, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Aldh5a1 ELISA Kit (Mouse) (OKEH05666) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Aldh5a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Aldh5a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Aldh5a1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 99.9%
Sensitivity
0.082 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Succinate-semialdehyde dehydrogenase, mitochondrial
Protein Length
523
NCBI Gene Symbol
Aldh5a1
Protein Name
Q8BWF0
Gene Name URL
Aldh5a1
Nucleotide Accession Number
NM_172532.3
Frequently Asked Questions
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