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ECHS1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Enoyl-CoA hydratase, short chain 1

Gene Aliases

ECHS1D; enoyl Coenzyme A hydratase, short chain, 1, mitochondrial; Enoyl-CoA hydratase 1; enoyl-CoA hydratase, mitochondrial; enoyl-CoA hydratase, short chain, 1, mitochondrial; epididymis secretory sperm binding protein; SCEH; short chain enoyl-CoA hydratase; Short-chain enoyl-CoA hydratase.

Gene ID

1892

Accession Number

NP_004083.3

Reactivity

Homo sapiens, Human

Target

Enoyl-CoA hydratase, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ECHS1 ELISA Kit (Human) (OKEH05661) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ECHS1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for ECHS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ECHS1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 98%

Sensitivity

39 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Enoyl-CoA hydratase, mitochondrial

Protein Length

290

NCBI Gene Symbol

ECHS1

Protein Name

P30084

Gene Name URL

ECHS1

Nucleotide Accession Number

NM_004092.3

Frequently Asked Questions

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