TM4SF20 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
Gene Name
Transmembrane 4 L six family member 20
Gene Aliases
1810018L02Rik; transmembrane 4 L6 family member 20.
Gene ID
66261
Accession Number
NP_079729.1
Reactivity
Mouse, Mus musculus
Target
Transmembrane 4 L6 family member 20
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tm4sf20 ELISA Kit (Mouse) (OKEH05650) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tm4sf20 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Tm4sf20 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tm4sf20 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 111.4%
Sensitivity
0.166 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Transmembrane 4 L6 family member 20
Protein Length
226
NCBI Gene Symbol
Tm4sf20
Protein Name
Q9CQY8
Gene Name URL
Tm4sf20
CAS Number
7732-18-5
Nucleotide Accession Number
NM_025453.3
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
