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SRSF3 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Serine and arginine-rich splicing factor 3

Gene Aliases

AL024116; pre-mRNA-splicing factor SRP20; Protein X16; serine/arginine-rich splicing factor 3; Sf; Sfrs3; Splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich 3 (SRp20) ; X16.

Gene ID

20383

Accession Number

NP_038691.1

Reactivity

Mouse, Mus musculus

Target

Serine/arginine-rich splicing factor 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Srsf3 ELISA Kit (Mouse) (OKEH05645) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Srsf3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Srsf3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Srsf3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 85.2%

Sensitivity

0.061 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Serine/arginine-rich splicing factor 3

Protein Length

164

NCBI Gene Symbol

Srsf3

Protein Name

P84104

Gene Name URL

Srsf3

Nucleotide Accession Number

NM_013663.5

Frequently Asked Questions

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