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LGR4 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Leucine-rich repeat-containing G protein-coupled receptor 4

Gene Aliases

9130225G07; A330106J01Rik; A930009A08Rik; G protein-coupled receptor GPR48; Gpr; Gpr48; G-protein coupled receptor 48; leucine-rich repeat-containing G-protein coupled receptor 4.

Gene ID

107515

Accession Number

NP_766259.2

Reactivity

Mouse, Mus musculus

Target

Leucine-rich repeat-containing G-protein coupled receptor 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Lgr4 ELISA Kit (Mouse) (OKEH05644) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lgr4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Lgr4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lgr4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.625-40ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 100.9%

Sensitivity

0.327 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Leucine-rich repeat-containing G-protein coupled receptor 4

Protein Length

951

NCBI Gene Symbol

Lgr4

Protein Name

A2ARI4

Gene Name URL

Lgr4

Nucleotide Accession Number

NM_172671.2

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