PDE10A ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
Gene Name
Phosphodiesterase 10A
Gene Aliases
CAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A; dual-substrate cAMP and cGMP, cyclic nucleotide phosphodiesterase.
Gene ID
23984
Accession Number
NP_001277636.1
Reactivity
Mouse, Mus musculus
Target
CAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Pde10a ELISA Kit (Mouse) (OKEH05641) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pde10a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Pde10a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pde10a captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 88.1%
Sensitivity
0.158 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
Protein Length
790
NCBI Gene Symbol
Pde10a
Protein Name
Q8CA95
Gene Name URL
Pde10a
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001290707.1
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