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PDE10A ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Phosphodiesterase 10A

Gene Aliases

CAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A; dual-substrate cAMP and cGMP, cyclic nucleotide phosphodiesterase.

Gene ID

23984

Accession Number

NP_001277636.1

Reactivity

Mouse, Mus musculus

Target

CAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Pde10a ELISA Kit (Mouse) (OKEH05641) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pde10a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Pde10a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pde10a captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 88.1%

Sensitivity

0.158 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A

Protein Length

790

NCBI Gene Symbol

Pde10a

Protein Name

Q8CA95

Gene Name URL

Pde10a

Nucleotide Accession Number

NM_001290707.1

Frequently Asked Questions

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