SERPINB5 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Serine (or cysteine) peptidase inhibitor, clade B, member 5
Gene Aliases
1110036M19Rik; AI462524; AI646751; M; Maspin; ova; ovalbumin; peptidase inhibitor 5; PI-5; protease inhibitor 5; S; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; serine (or cysteine) proteinase inhibitor, clade B, member 5; serine protease inhibitor 7; serpin B5; Spi7.
Gene ID
20724
Accession Number
NP_033283.1
Reactivity
Mouse, Mus musculus
Target
Serpin B5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Serpinb5 ELISA Kit (Mouse) (OKEH05628) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpinb5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Serpinb5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serpinb5 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 81%
Sensitivity
0.427 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Serpin B5
Protein Length
375
NCBI Gene Symbol
Serpinb5
Protein Name
P70124
Gene Name URL
Serpinb5
Nucleotide Accession Number
NM_009257.3
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