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P2RX7 ELISA Kit (Mouse)

Selected Variant

OKEH05605-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Purinergic receptor P2X, ligand-gated ion channel, 7

Gene Aliases

AI467586; ATP receptor; P2X; P2X purinoceptor 7; P2X (7; P2X (7) ; P2X7 purinoceptor; P2X7 receptor; P2X7R; P2Z receptor; Purinergic receptor.

Gene ID

18439

Accession Number

NP_035157.2

Reactivity

Mouse, Mus musculus

Target

P2X purinoceptor 7

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology P2rx7 ELISA Kit (Mouse) (OKEH05605) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for P2rx7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for P2rx7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample P2rx7 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 92%

Sensitivity

0.083 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse P2X purinoceptor 7

Protein Length

595

NCBI Gene Symbol

P2rx7

Protein Name

Q9Z1M0

Gene Name URL

P2rx7

Nucleotide Accession Number

NM_011027.3

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