What is the catalog number for CDK4 ELISA Kit (Mouse)?

The catalog number for CDK4 ELISA Kit (Mouse) is OKEH05604-96W.

What sizes are available for CDK4 ELISA Kit (Mouse)?

CDK4 ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is CDK4 ELISA Kit (Mouse) in stock?

CDK4 ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.

Do you offer bulk or custom quantities of this product?

Yes. Ordering two or more units, or combining this with other products, usually qualifies for a discount depending on quantity. Contact our sales team with your required volume for a custom quote.

CDK4 ELISA Kit (Mouse)

Selected Variant

OKEH05604-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Cyclin-dependent kinase 4

Gene Aliases

/cdk4; PSK-J3; serine/threonine kinase.

Gene ID

12567

Accession Number

NP_034000.1

Reactivity

Mouse, Mus musculus

Target

Cyclin-dependent kinase 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Cdk4 ELISA Kit (Mouse) (OKEH05604) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cdk4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cdk4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cdk4 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 90.7%

Sensitivity

39.2 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Cyclin-dependent kinase 4

Protein Length

303

NCBI Gene Symbol

Cdk4

Protein Name

P30285

Gene Name URL

Cdk4

Nucleotide Accession Number

NM_009870.3

Price

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