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ATP2B2 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

ATPase plasma membrane Ca2+ transporting 2

Gene Aliases

ATPase, Ca++ transporting, plasma membrane 2; plasma membrane Ca (2+) -ATPase; plasma membrane Ca2+ pump 2; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 2; plasma membrane calcium pump; Plasma membrane calcium pump isoform 2; plasma membrane calcium-transporting ATPase 2; PMCA2; PMCA2a; PMCA2i.

Gene ID

491

Accession Number

NP_001001331.1

Reactivity

Homo sapiens, Human

Target

Plasma membrane calcium-transporting ATPase 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ATP2B2 ELISA Kit (Human) (OKEH05593) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATP2B2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATP2B2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATP2B2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 96%

Sensitivity

0.082 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Plasma membrane calcium-transporting ATPase 2

Protein Length

1243

NCBI Gene Symbol

ATP2B2

Protein Name

Q01814

Gene Name URL

ATP2B2

Nucleotide Accession Number

NM_001001331.2

Frequently Asked Questions

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