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LAMA3 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Laminin, alpha 3

Gene Aliases

[a]3; [a]3B; epiligrin subunit alpha; kalinin subunit alpha; Lama3B; laminin subunit alpha-3; laminin-5 subunit alpha; laminin-6 subunit alpha; laminin-7 subunit alpha; nicein subunit alpha; nicein, 150kDa.

Gene ID

16774

Accession Number

NP_034810.1

Reactivity

Mouse, Mus musculus

Target

Laminin subunit alpha-3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Lama3 ELISA Kit (Mouse) (OKEH05552) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lama3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lama3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lama3 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates, urine, cell culture supernatants and other biological fluids

Detection Range

1.56-100ng/mL

Sensitivity

0.797 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Laminin subunit alpha-3

Protein Length

3330

NCBI Gene Symbol

Lama3

Protein Name

Q61789

Gene Name URL

Lama3

Nucleotide Accession Number

NM_010680.1

Frequently Asked Questions

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