What is the catalog number for ALOX8 ELISA Kit (Mouse)?

The catalog number for ALOX8 ELISA Kit (Mouse) is OKEH05541-96W.

What sizes are available for ALOX8 ELISA Kit (Mouse)?

ALOX8 ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is ALOX8 ELISA Kit (Mouse) in stock?

ALOX8 ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

ALOX8 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Arachidonate 8-lipoxygenase

Gene Aliases

15-lipoxygenase 2;15-LOX-2;15-LOX-B;8-L;8-LOX;8S-;8S-lipoxygenase;8S-LOX; Alox; Alox15b; arachidonate 15-lipoxygenase B; arachidonate 15-lipoxygenase type II; arachidonate 15-lipoxygenase, second type; arachidonate 8S-lipoxygenase; linoleate 9S-lipoxygenase ALOX8; polyunsaturated fatty acid lipoxygenase ALOX8.

Gene ID

11688

Accession Number

NP_033791.1

Reactivity

Mouse, Mus musculus

Target

Arachidonate 8S-lipoxygenase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Alox8 ELISA Kit (Mouse) (OKEH05541) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Alox8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Alox8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alox8 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol